Immunization against Ehrlich Mouse Ascites Carcinoma with Chemically Devitalized Tumor Cells.

吉村 政弘 ・蕪城 外枝子 * This work was supported , in part, by grants from the Japan Anti-Cancer Society, the National Institute of Health, U. S. A., and the Tokyo Biochemistry Research Fund. Attempts to induce immunity against Ehrlich ascites carcinoma in inbred mice have been the subject of considerable studies in recent years (1-4). Various procedures were employed with varying degrees of success to prepare altered cancer cells which would be effective in producing resistance to subsequent transplant of the carcinoma cells. McKee et al. (5) failed to induce immunity by pretreatment of mice with Ehrlich ascites cells that had been killed by desiccation, freezing and thawing, mechanical grinding, and supersonic waves. Revesz (6), and Donaldson and Mitchell (7) were successful to protect mice by immunization with X-irradiated carcinoma cells. Recently, Donaldson and North (8) demonstrated that repeated injections of tumor cells inactivated with nitrogen mustard resulted in a high degree of immunity. The present report deals with attempts to induce immunity in dd strain of mice against Ehrlich ascites carcinoma by use of cells treated with various chemical agents.